(Download) "Molecular Identification of the Economically Important Invasive Citrus Root Weevil Diaprepes Abbreviatus (Coleoptera: Curculionidae) (Scientific Notes) (Report)" by Florida Entomologist " eBook PDF Kindle ePub Free
eBook details
- Title: Molecular Identification of the Economically Important Invasive Citrus Root Weevil Diaprepes Abbreviatus (Coleoptera: Curculionidae) (Scientific Notes) (Report)
- Author : Florida Entomologist
- Release Date : January 01, 2009
- Genre: Life Sciences,Books,Science & Nature,
- Pages : * pages
- Size : 214 KB
Description
Diaprepes abbreviatus L. is a polyphagous weevil affecting more than 270 species of plants. The larvae feed on roots of the trees causing damage that can kill the plant. Originally from the Caribbean, this weevil was first found in the United States in Apopka, Florida in 1964 (Woodruff 1964) and it currently infests 23 counties in Florida. In 2000, D. abbreviatus was discovered in the Rio Grande Valley, Texas (Skaria & French 2001) and in 2005 in southern California (California Department of Food and Agriculture, CDFA, 2007). Different control measurements, which are specific to a particular life stage of the weevil, are currently in use (McCoy et al. 2007). However, when egg masses or larvae are found, the diagnosis of infestation is often delayed due to lack of reliable methods that allow one to identify nonadult stages. The objective of this study was to develop a method for species identification of immature stages of D. abbreuiatus based on DNA barcoding technique. DNA barcoding consists of sequencing of a DNA segment from a specified region of the genome, and the "barcode" sequences are compared to those available in a reference database to determine the species represented by the sample. The mitochondrial gene cytochrome oxidase I (COI) is extensively used for barcoding of metazoans (Hebert et al. 2003). Moreover, COI sequences are the most commonly used for quarantine and forensic applications involving insects, Tetranychus mites (Lee & Lee 1997), Liriomyza leafminers (Scheffer et al. 2001) and Calliphora blowflies (Ames et al. 2006). In this study, a reliable method based on PCR and sequencing of the COI gene was developed to identify immature specimens of D. abbreviatus and to differentiate them from another common root weevil, Pantomorus ceruinus (Boheman) Kuschel.
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