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Molecular Diagnosis of Mycobacteria (Minireview)

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eBook details

  • Title: Molecular Diagnosis of Mycobacteria (Minireview)
  • Author : Clinical Chemistry
  • Release Date : January 01, 2001
  • Genre: Chemistry,Books,Science & Nature,
  • Pages : * pages
  • Size : 180 KB

Description

The genus Mycobacterium consists of the members of the Mycobacterium tuberculosis complex and 80 species of nontuberculous mycobacteria, including pathogenic, opportunistic, and nonpathogenic species. The most important species of this genus is M. tuberculosis, the causative agent of tuberculosis (TB).4 TB is one of the leading infectious diseases in the world and is responsible for more than 2 million deaths and 8 million new cases annually (1). M. avium is the most common nontuberculous mycobacterium found in clinical specimens. Infections caused by this species can be clinically significant, especially when associated with AIDS (2). The major difference between TB and other mycobacterial infections is that M. tuberculosis is transmitted from person to person. Thus it is particularly important to diagnose TB as rapidly as possible. In addition, antibiotic treatment varies according to the species of mycobacterium. Although the initial diagnosis of mycobacterial disease often is based on clinical data, definitive diagnosis usually involves the isolation and identification of the infecting organism in the laboratory. The usual laboratory procedure for clinical specimens involves decontamination and digestion of the specimen, microscopic examination for the presence of acid-fast bacilli (AFB), isolation of the organism by culture, and identification and drug susceptibility testing of the recovered organism. Because of the slow growth rate of mycobacteria, isolation, identification, and drug susceptibility testing can take several weeks or longer.


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