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eBook details

• Title: Molecular Diagnostics of Infectious Diseases.
• Author : Clinical Chemistry
• Release Date : January 01, 1997
• Genre: Chemistry,Books,Science & Nature,
• Pages : * pages
• Size : 256 KB

Description

Over the past century microbiologists have searched for more rapid and efficient means of microbial identification. The identification and differentiation of microorganisms has principally relied on microbial morphology and growth variables. Advances in molecular biology over the past 10 years have opened new avenues for microbial identification and characterization [1-5]. The traditional methods of microbial identification rely solely on the phenotypic characteristics of the organism. Bacterial fermentation, fungal conidiogenesis, parasitic morphology, and viral cytopathic effects are a few phenotypic characteristics commonly used. Some phenotypic characteristics are sensitive enough for strain characterization; these include isoenzyme profiles, antibiotic susceptibility profiles, and chromatographic analysis of cellular fatty acids [6-13]. However, most phenotypic variables commonly observed in the microbiology laboratory are not sensitive enough for strain differentiation. When methods for microbial genome analysis became available, a new frontier in microbial identification and characterization was opened.

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